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- Title
Essential roles of Gα12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements.
- Authors
Lin, Fang; Sepich, Diane S.; Chen, Songhai; Topczewski, Jacek; Yin, Chunyue; Solnica-Krezel, Lilianna; Hamm, Heidi
- Abstract
Gα12/13 have been implicated in numerous cellular processes, however, their roles in vertebrate gastrulation are largely unknown. Here, we show that during zebrafish gastrulation, suppression of both Gα12 and Gα13 signaling by over expressing dominant negative proteins and application of antisense morpholino-modified oligonucleotide translation interference disrupted convergence and extension without changing embryonic patterning. Analyses of mesodermal cell behaviors revealed that Gα12/13 are required for cell elongation and efficient dorsalward migration during convergence independent of non canonical Wnt signaling. Furthermore, Gα12/13 function cell-autonomously to mediate mediolateral cell elongation underlying intercalation during notochord extension, likely acting in parallel to non canonical Wnt signaling. These findings provide the first evidence that Gα12 and Gα13 have overlapping and essential roles in distinct cell behaviors that drive vertebrate gastrulation.
- Subjects
GASTRULATION; STOCHASTIC convergence; ZEBRA danio; PROTEINS; OLIGONUCLEOTIDES; EMBRYOLOGY
- Publication
Journal of Cell Biology, 2005, Vol 169, Issue 5, p777
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200501104