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- Title
Reprogramming an RNA-guided archaeal TnpB endonuclease for genome editing.
- Authors
Xu, Ying; Liu, Tao; Wang, Jing; Xiong, Binyang; Liu, Ling; Peng, Nan
- Abstract
This article discusses the reprogramming of an RNA-guided archaeal TnpB endonuclease, SisTnpB1, for genome editing. The researchers investigated the functions of TnpB from Sulfolobus islandicus and found that it exhibited genome editing activity in human cells. They cloned the SisTnpB1 gene and its ωRNA coding sequence and conducted in vitro DNA cleavage assays, showing that SisTnpB1 cleaved target plasmid DNA in a sequence-specific manner. The researchers also demonstrated the efficiency of in vivo DNA interference and genome editing using SisTnpB1 in Pediococcus acidilactici LA412. Overall, SisTnpB1 shows potential as a programmable endonuclease for genome editing in different organisms living at different temperatures.
- Subjects
GENOME editing; ENDONUCLEASES; PEDIOCOCCUS acidilactici; RESEARCH personnel; MOLECULAR cloning; CODES of ethics
- Publication
Cell Discovery, 2023, Vol 9, Issue 1, p1
- ISSN
2056-5968
- Publication type
Article
- DOI
10.1038/s41421-023-00615-2