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- Title
Interleukin-17A enhances osteogenic differentiation through the activation of OPG/RANKL and ERK/MAPK signalling pathways in stem cells from human exfoliated deciduous teeth.
- Authors
Abd Mutalib, Mohd Nor Ridzuan; Sebastian, Alphy Alphonsa; Noorazman, Arin Afifa; Kannan, Thirumulu Ponnuraj; Abdullah, Nurul Asma
- Abstract
Introduction: In tissue regenerative procedures, multipotent stem cells derived from human exfoliated deciduous teeth (SHED) represent a promising cell source. Interleukin-17A (IL-17A) have a potential role in promoting osteogenic differentiation of SHED. Herein, we investigated the stimulative effects of IL-17A on osteogenic differentiation in SHED through MAPK and OPG/RANKL signalling pathways and the possible role of miRNA-145 (miR-145) in osteogenesis of SHED. Method: SHED were cultured in two conditions; 1) SHED in complete alpha minimum essential medium (α-MEM); 2) SHED in complete α-MEM supplemented with osteogenic reagents and treated +/- with recombinant IL-17A. For osteogenic differentiation analysis, the expression of the miRNA-145 was quantified using real-time PCR; and the expression of specific protein markers was analyzed by Western blot. The effect on the MAPK signalling pathway was assessed by RT2 profiler PCR array and confirmed using ERK/MAPK antibodies by western blotting. Results: This work showed that the expression levels of several osteogenic markers such as ALP, COL1A1, RUNX2, OCN, OPN, OPG and OPG/RANKL ratio were significantly increased in IL-17A-treated SHED (P < 0.01). Besides, the expression of miR-145 was also up-regulated in both groups by IL-17A treatment. In addition, the result showed that IL-17A activates the MAPK signalling pathway in SHED cultured in both culture conditions by the significant upregulation of all upstream activators and downstream targets of ERK, P38 and JNK pathways. The activation of MAPK signalling was confirmed by ERK/MAPK, which was activated during the osteogenic differentiation of SHED in a time-dependent manner. Conclusion: These findings demonstrate that the activation of OPG/RANKL and MAPK signalling pathways and miR-145 by IL-17A is essential for osteogenic differentiation of SHED, which reiterates the significance of these intracellular signalling pathways and microRNA in regulating the osteogenic differentiation of SHED; which is an alternative cell source for bone tissue engineering.
- Subjects
CELLULAR signal transduction; DECIDUOUS teeth; HUMAN stem cells; MITOGEN-activated protein kinases; TRANCE protein
- Publication
Malaysian Journal of Medicine & Health Sciences, 2022, Vol 18, p20
- ISSN
1675-8544
- Publication type
Abstract