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- Title
Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology.
- Authors
Zhang, Chao; Zheng, Xin; Zhao, Chengna; Li, Yan; Chen, Shuiping; Liu, Gang; Wang, Chengbin; Lv, Qingyu; Liu, Peng; Zheng, Yuling; Kong, Decong; Jiang, Hua; Jiang, Yongqiang
- Abstract
Bloodstream infections (BSIs) are often life-threatening, and rapid identification is critical. Here, we developed a TaqMan array card (TAC) assay to detect pathogens in BSI specimens. The TAC included 30 primer/probe pairs targeting 27 species and 3 controls. Reverse transcription and 0.1% blue dextran 2000 increased the TAC assay efficiency. The primer/probe pairs had a limit of detection of 100-102 CFU/mL and a specificity of 100%. For whole blood specimens, the TAC assay showed a sensitivity and specificity of 79.4% and 99.69%, respectively. For blood culture samples, the TAC assay showed a sensitivity and specificity of 100% and 99.67%, respectively. The TAC assay could be a promising method for early detection of bloodstream infection.
- Publication
Scientific Reports, 2018, Vol 8, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-018-31200-3