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- Title
Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi.
- Authors
Martins Silva, Aline Lourdes; Ferreira Santana, Marcos Diones; de Jesus Pereira, John Cesar; Pupo Raimam, Milena; Brigatto Albino, Ulisses
- Abstract
Arbuscular mycorrhizae microscopy requires differential staining of typical structures. Dyes employed, such as trypan blue, pose risks to health and environment. Alternative dyes such as pen ink and aniline have variable coloring efficiency. In this work, Brachiaria decumbens roots, discolored with caustic soda (NaOH), were stained with açai, annatto, saffron, trypan blue and pen inks. There were significant differences among dyes regarding stained mycorrhizal structures and pictures quality. Acai was considered the best alternative dye, with similar results to trypan blue.
- Subjects
VESICULAR-arbuscular mycorrhizas; ENDOMYCORRHIZAS; TRYPAN blue; MICROSCOPY; BRACHIARIA decumbens; FOOD preservation
- Publication
Brazilian Journal of Forest Research / Pesquisa Florestal Brasileira, 2015, Vol 35, Issue 84, p475
- ISSN
1809-3647
- Publication type
Article
- DOI
10.4336/2015.pfb.35.84.798