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- Title
Four cytosolic-type CuZn-superoxide dismutases in germinating seeds of Pinus sylvestris.
- Authors
Stroller, Steflen; Karpinski, Stanislaw; Hällgren, Jan-Erik; Wingsle, Gunnar
- Abstract
A differential analysis of CuZn-superoxide dismutase (SOD. EC 1.15.1.1) isozymes after native-polyacrylamide gel electrophoresis (PAGE) and isoelectric focusing (IEF) indicated that germinating seeds of Scots pine (Pinus sylvestris L.) 3 days after the start of imbibition (3 DAI) contain five CuZn-SOD isozymes. Two isozymes co-migrated on native-PAGE but were separated after IEF CuZn-SODS of Scots pine were purified from germinating seeds (3 DAI) by anion-exchange chromatography, hydrophobic interaction chromatography and chromatofocusing. The final separation of CuZn-SOD isozymes was accomplished by native-PAGE. CuZn-SOD isozymes were electroblotted and their NH2terminal amino acid sequence was determined. Comparisons of the amino acid sequences with sequences of CuZn-SOD isozymes from other plant sources indicated that one CuZn-SOD isozyme was of the chloroplastic type whereas the other four isozymes belonged to the cytosolic-type CuZn-SODs. The NH2-terminal amino acid sequence of the chloroplastic CuZn-SOD and of one cytosolic-type CuZn-SOD were identical to those of two previously isolated, sequenced and localized CuZn-SOD isozymes from Scots pine needles. Two cytosolic-type CuZn-SOD isozymes showed a homology at 20 out of 21 NH2-terminal amino acids. Mitochondria and glyoxysomes were isolated by differential and Percoll density-gradient centrifugation from germinating seeds (3 DAI). The cell fractionation experiments did not suggest that a major part of the CuZn-SOD activity in germinating seeds was derived from glyoxysomes or mitochondria.
- Subjects
GLYOXYSOMES; ORGANELLES; SEEDS; SCOTS pine; PLANT physiology; ISOENZYMES; GERMINATION
- Publication
Physiologia Plantarum, 1994, Vol 92, Issue 3, p443
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1994.tb08834.x