We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Induced Ca<sup>2+</sup> uptake and callose synthesis in suspension-cultured cells of Catharanthus roseus are decreased by the protein phosphatase inhibitor okadaic acid.
- Authors
Kauss, Heinrich; Jeblick, Wolfgang
- Abstract
Subtoxic concentrations of the saponin digitonin the polyene antibiotic amphotericin B and the bacterial phytotoxin syringomycin induce increased uptake of 45Ca2+ into suspension-cultured plant cells and a rapid Ca2+-dependent defense response, callose synthesis. Both reactions were inhibited by preincubation of the cells with okadaic acid, a specific inhibitor of type 1 and type 2A protein phosphatases. These results suggest that Ca2+ uptake induced by the above agents does not occur due to unspecific perturbation of plasma membrane permeability but involves transport proteins which are controlled by protein phosphorylation/dephosphorylation. Phosphoproteins appear also to be involved in the regulation of callose synthesis, although it remains open whether this control is effected at the level of Ca2+ transport or at the 1,3-β-glucan synthase involved in deposition of the polymer.
- Subjects
CATHARANTHUS roseus; PLANT cells &; tissues; APOCYNACEAE; PROTEINS; PHOSPHATES; CATHARANTHUS
- Publication
Physiologia Plantarum, 1991, Vol 81, Issue 3, p309
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1991.tb08737.x