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- Title
Spin‐coated ZnO–graphene nanostructure thin film as a promising matrix for urease immobilization of impedimetric urea biosensor.
- Authors
Ghayedi Karimi, Kobra; Mozaffari, Sayed Ahmad; Ebrahimi, Mahmoud
- Abstract
The fabrication, characterization, and application of a nanostructured zinc oxide/graphene (ZnO/Gr) transducer were described for the determination of urea concentration. For this purpose, thin films of sol–gel‐synthesized ZnO nanostructure and Gr were prepared using the spin coating technique on the conductive fluorinated–tin oxide (FTO) substrate. Appropriate correlations between the spinning parameters and thin film features were established so that an efficient ZnO–Gr thin film was obtained. Field emission scanning electron microscopy (FE‐SEM) images prove thin film pores to be an operative biosensing zone for urease (Urs) enzyme immobilization. Step‐by‐step monitoring of FTO/ZnO‐Gr/Urs biosensor fabrication was achieved using electrochemical methods. The impedimetric results of a fabricated FTO/ZnO‐Gr/Urs biosensor show high sensitivity for urea detection within 5.0–125.0 mg/dL and a limit of detection of 3.36 mg/dL. In our research group in the thin film and nanotechnology laboratory, we are working on the development of semiconductor‐based transducers for enzymatic biosensors and impedimetric assessment for biosensing. This work reports a novel transducer using spin‐coated ZnO–graphene nanostructure thin film on FTO surface and its effects on the improvement of enzymatic urea biosensing.
- Subjects
SPIN coating; ZINC oxide; GRAPHENE; NANOSTRUCTURED materials; METALLIC thin films; UREASE; BIOSENSORS
- Publication
Journal of the Chinese Chemical Society, 2018, Vol 65, Issue 11, p1379
- ISSN
0009-4536
- Publication type
Article
- DOI
10.1002/jccs.201800031