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- Title
Components of the peptidoglycan-recycling pathway modulate invasion and intracellular survival ofSalmonella entericaserovar Typhimurium.
- Authors
Folkesson, Anders; Eriksson, Sofia; Andersson, Mats; Park, James T.; Normark, Staffan
- Abstract
β-Lactam resistance in enteric bacteria is frequently caused by mutations inampDencoding a cytosolicN-acetylmuramyl-l-alanine amidase. Such mutants are blocked in murein (peptidoglycan) recycling and accumulate cytoplasmic muropeptides that interact with the transcriptional activatorampR,which de-repressesβ-lactamase expression.Salmonella entericaserovar Typhimurium,an extensively studied enteric pathogen, was used to show that mutations inampDdecreased the ability ofS. typhimuriumto enter a macrophage derived cell line and made the bacteria more potent as inducers of inducible nitric oxide synthase (iNOS), as compared with the wild-type.ampGmutants, defective in the transport of recycled muropeptides across the cytoplasmic membrane, behaved essentially as the wild-type in invasion assays and in activation of iNOS. AsampDmutants also have reducedin vivofitness in a murine model, we suggest that the cytoplasmic accumulation of muropeptides affects the virulence of theampDmutants.
- Subjects
PEPTIDOGLYCANS; SALMONELLA; GENETIC mutation; BACTERIA; CELL lines; NITRIC oxide
- Publication
Cellular Microbiology, 2005, Vol 7, Issue 1, p147
- ISSN
1462-5814
- Publication type
Article
- DOI
10.1111/j.1462-5822.2004.00443.x