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- Title
Protective immunization of calves against Ostertagia ostertagi using fourth stage larval extracts.
- Authors
HALLIDAY, A. M.; SMITH, W. D.
- Abstract
ConA lectin was used to isolate glycoproteins from detergent extracts of fourth stage Ostertagia ostertagi larvae. This preparation contained proteins additional to those observed in a similar fraction prepared from adult O. ostertagi. Two vaccine trials were conducted with this preparation, and sub-fractions thereof, in groups of 6–8 worm-free calves. All groups were challenged with 50 000 O. ostertagi larvae 1 week after the final immunization, and protection was assessed by comparing the egg and worm counts of the immunized groups with their respective controls. Immunization with the ConA-binding antigen or its sub-fractions induced high titre serum antibody responses. In the first trial, the cumulative egg count of the group immunized with unfractionated antigen was 60% lower than the corresponding control value, and worm counts were 47% lower. In the second trial, the cumulative egg counts of the vaccinated groups ranged from 70% to 85% lower than the corresponding controls, with worm counts up to 64% lower. It was concluded that detergent-soluble, ConA-binding extracts prepared from O. ostertagi fourth stage larvae contained protective immunogens that were as effective as the best antigens published for O. ostertagi to date.
- Subjects
CALVES; CATTLE diseases; IMMUNIZATION; GLYCOPROTEINS; LECTINS; EXTRACTS
- Publication
Parasite Immunology, 2010, Vol 32, Issue 9/10, p656
- ISSN
0141-9838
- Publication type
Article
- DOI
10.1111/j.1365-3024.2010.01221.x