We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of Combined Live Bifidobacterium , Lactobacillus , Enterococcus , and Bacillus Cereus Tablets on the Structure and Function of the Intestinal Flora in Rabbits Undergoing Hepatic Artery Infusion Chemotherapy.
- Authors
Yan, Xiangdong; Bai, Liuhui; Lv, Jin; Qi, Ping; Song, Xiaojing; Zhang, Lei
- Abstract
Simple Summary: Patients with hepatocellular carcinoma (HCC) often have a variety of adverse reactions after chemotherapy, which may be related to the changes in intestinal flora caused by chemotherapy. In this study, a rabbit model of liver local arterial perfusion chemotherapy was established. The effects of Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets on the structure of intestinal microbiota and intestinal barrier function in rabbits treated with local arterial perfusion chemotherapy were studied, and the possible potential mechanisms were discussed. This study provides a theoretical basis for probiotics to regulate intestinal microbiota and improve the side effects of local arterial perfusion chemotherapy drugs in the liver. After arterial chemotherapy with epirubicin, the relative abundance of beneficial bacteria, such as Cladosporium and Bacteroides, decreased, while the relative abundance of harmful bacteria, such as Proteus, increased. In rabbit serum and ileum tissue, the protein expression levels of the intestinal barrier tight-junction proteins ZO-1, occludin, and claudin-1 decreased, while the levels of inflammatory factors and liver injury factors increased. Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets can reverse the composition of intestinal flora. The relative abundance of beneficial bacteria, such as rabbit Bifidobacterium, rumen coccus, Prevost's bacteria, and Blautia, increased, while the relative abundance of Proteus, verrucous microorganisms, Escherichia/Shigella, and Sporobacter decreased. It also significantly increased the expression of the rabbit tight-junction proteins ZO-1, occludin, and claudin-1, increased the expression of mucin gene, reduced intestinal permeability, and repaired intestinal barrier injury. Probiotic regulation reduces the expression levels of inflammatory factors and liver injury factors in rabbit serum. The correlation analysis between intestinal flora and clinical environmental factors showed that beneficial bacteria were positively correlated with the protein expression levels of the intestinal tight-junction proteins claudin-1, ZO-1, and occludin, while beneficial bacteria were negatively correlated with harmful bacteria. The expression level of beneficial bacteria was negatively correlated with inflammatory factors and liver injury factors, and positively correlated with harmful bacteria. Few studies have explored the biological mechanism by which probiotics alleviate adverse reactions to chemotherapy drugs after local hepatic chemotherapy perfusion by regulating the intestinal flora. This study investigates the effects of Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets on the intestinal microbial structure and intestinal barrier function, as well as the potential mechanism in rabbits after local hepatic chemotherapy infusion. Eighteen New Zealand White rabbits were randomly divided into a control group, a hepatic local chemotherapy perfusion group, and a hepatic local chemotherapy perfusion + Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets group to assess the effects of Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets on the adverse reactions. The administration of Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets alleviated the intestinal flora disorder caused by local hepatic perfusion chemotherapy, promoted the growth of beneficial bacteria, and inhibited the growth of harmful bacteria. The Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets also reduced the levels of serum pro-inflammatory cytokines and liver injury factors induced by local hepatic perfusion chemotherapy. Our findings indicate that Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets can ameliorate the toxicity and side effects of chemotherapy by regulating intestinal flora, blocking pro-inflammatory cytokines, reducing liver injury factors, and repairing the intestinal barrier. Probiotics may be used as a potential alternative therapeutic strategy to prevent the adverse reactions caused by chemotherapy with local hepatic perfusion.
- Subjects
BACILLUS cereus; BIFIDOBACTERIUM; HEPATIC artery; CHEMOTHERAPY complications; INTESTINAL barrier function; ENTEROCOCCUS
- Publication
Biology (2079-7737), 2024, Vol 13, Issue 5, p327
- ISSN
2079-7737
- Publication type
Article
- DOI
10.3390/biology13050327