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- Title
Media optimization for lipase from Halomonas salina, a moderately halophilic bactrium isolated from excreta of wild Ass.
- Authors
Khunt, Mrugesh; Pandhi, Neepa
- Abstract
Twenty-four moderate halophiles were isolated from excreta of wild Ass after enrichment in complete media broth and halophilic broth. All the isolates were Gram's positive and non-capsulated. Further, isolates were screened for extracellular lipase secretion on solid media containing tributyrin as carbon source. Halomonas salina was the potential lipase producer identified on the basis of 16s r-RNA sequencing and further optimized for lipase secretion in liquid media. Maximum lipase secretion was after 264 hours of incubation i.e. during stationary phase. Isolates were found to secrete maximum lipase at pH 5, 14% NaCl, 30- 40°C temperature and 3% substrate concentration.
- Subjects
LIPASES; HALOPHILIC organisms; NUCLEOTIDE sequence; MICROBIOLOGY of extreme environments; HYDROLYSIS
- Publication
International Journal of Pharmacy & Life Sciences, 2012, Vol 3, Issue 1, p1351
- ISSN
0976-7126
- Publication type
Article