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- Title
Use of two different methods for glucose determination in sheep under normoglycemic, hypoglycemic, and hyperglycemic conditions: an evaluation of practical diagnostic methods in ovines.
- Authors
Chenard, Marina Galindo; Bastos, Jade de Menezes Paes; Valle, Matheus Dias; Magalhães da Cunha, Isabelle; Alencar, Nayro Xavier de; Lessa, Daniel Augusto Barroso; Souza, Guilherme Nunes de; Cunha, Nathalie Costa da; Helayel, Michel Abdalla
- Abstract
Context: Animals can present abnormal blood glucose concentrations because of various diseases or pathological conditions, stress, or hunger. Early diagnosis prevents complications, economic losses, and death. The use of a portable glucometer (PGM) has been shown to be a good, simple, and practical alternative method with good precision and accuracy for assessing blood glucose in humans and companion animals. Aims: The objective of this work was to evaluate the accuracy and reliability of a portable glucometer (PGM) for assessing glycemia in normoglycemic, hypoglycemic, and hyperglycemic sheep. Methods: Blood glucose was evaluated in 60 normoglycemic, 15 hypoglycemic, and 15 hyperglycemic sheep. Blood samples were collected and analysed within 2 h by using PGM and the enzymatic method (EM). Each test was evaluated for sensitivity, specificity, and the area under the receiver operating characteristic (ROC) curve for two cutoff points, namely, one for hypoglycemia and the other for hyperglycemia. Key results: The results of the Kolmogorov–Smirnov test (P < 0.05) for all groups evaluated did not show a normal distribution for the values evaluated by PGM and EM. Despite the significant difference found between the medians of the methods and the low homogeneity according to the coefficient of variation (CV), there was a homogeneous and linear dispersion of the results. The Bland–Altman test showed that the mean difference between the two methods was close to zero, denoting good agreement, precision, and accuracy of PGM when compared to EM. Conclusions: PGM presents high accuracy and precision for assessing glycemia in sheep, providing satisfactory and reliable results when compared with EM. Implications: The use of PGM facilitates the veterinarian's routine, promoting early diagnosis, field examinations, and monitoring of metabolic diseases. The assessment of glycemia is crucial for diagnosing pathologies, balancing diets and measuring stress in production animals. Portable glucometer (PGM) has been shown to be an effective, simple, practical and accessible tool for measuring blood glucose in goats, cattle and horses, for example. Here we used PGM to assess glycemia in sheep, and found that it provides satisfactory and reliable results when compared with enzymatic method. The use of PGM promotes early diagnosis, field tests and monitoring of metabolic diseases.
- Subjects
BLOOD sugar; RECEIVER operating characteristic curves; PETS; EFFECT of stress on animals; METABOLIC disorders; HYPERGLYCEMIA
- Publication
Animal Production Science, 2024, Vol 64, Issue 11, p1
- ISSN
1836-0939
- Publication type
Article
- DOI
10.1071/AN24165