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- Title
Cryoprotective effect of glycerol concentrations on Indian Red Jungle Fowl (Gallus gallus murghi) spermatozoa.
- Authors
Rakha, Bushra Allah; Ansari, Muhammad Sajjad; Akhter, Shamim; Blesbois, Elisabeth
- Abstract
Semen cryopreservation protocols for wild avian species need to be optimised in order to achieve optimum post-thaw sperm quality and fertility. The present study was designed to evaluate the cryoprotective effect of different glycerol concentrations (11%, 15% and 20%) on post-thaw quality, recovery rates, absolute livability index and fertility of Indian Red Jungle Fowl (Gallus gallus murghi) semen. Semen was collected from eight mature cocks and cryopreserved for storage at -196 °C. Frozen semen was thawed at 37 °C for 30 s and assessed for motility, plasma membrane integrity, viability and acrosome integrity at 0, 2 and 4 h incubation at 37 °C. Percentages of motility, plasma membrane integrity, viability and acrosome integrity were recorded higher (P<0.05) post-thaw at 0, 2 and 4 h at 37 °C with 20% glycerol compared to 15% and 11% glycerol. Likewise, recovery rates (%) of aforementioned parameters after cryopreservation and absolute livability index were observed highest (P<0.05) with 20% glycerol. By comparing values of R2 after multivariate regression analysis, least negative effects of hours of incubation were observed on semen quality in extenders with 20% glycerol followed by 15% and 11% glycerol. The fertility outcomes (number of fertile eggs, fertility [%], number of hatched chicks, percent hatch and hatchability of fertilised eggs) were recorded higher (P<0.05) with 20% glycerol followed by 15% and 11% glycerol. It is concluded that the concentration of 20% glycerol gives the best cryoprotection for quality and fertility of Indian Red Jungle Fowl semen.
- Subjects
JUNGLEFOWL; GLYCERIN; FOREST birds; BIRD behavior; BEHAVIOR evolution; SPERMATOZOA physiology; CRYOPRESERVATION of biological cultures; ANIMAL behavior; BIRDS
- Publication
Avian Biology Research, 2018, Vol 11, Issue 2, p80
- ISSN
1758-1559
- Publication type
Article
- DOI
10.3184/175815618X15180876264262