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- Title
Study on mass production of aquaporinZ for biomimetic water purification membrane.
- Authors
Sung-Jo Kim; Jinwook Lee; Hye-Weon Yu; In S. Kim
- Abstract
Aquaporin biomimetic membrane can only transfer water molecules in and out of the membrane while preventing the passage of other smaller ions and solutes. AquaporinZ (AqpZ), widely spread in Escherichia coli cell membrane, has shown higher water permeability than conventional membranes. Application of those exceptional properties as water purification membrane material is promising. The objective of this study was to assess protein expression conditions for AqpZ mass production. Recombinant AqpZ was successfully synthesized and identified via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blot analysis. The highest level of expression of his-tagged AqpZ (12.2 mg/L) was achieved using E. coli BL21(DE3) host strain by the addition of 0.1mM isopropyl-b-D-thiogalactoside and 5 h postinduction time. Subsequently, solubilization and purification of histagged AqpZ were carried out.
- Subjects
AQUAPORINS; ARTIFICIAL membranes; MEMBRANE filtration in water purification
- Publication
Desalination & Water Treatment, 2013, Vol 51, Issue 31-33, p6370
- ISSN
1944-3994
- Publication type
Abstract
- DOI
10.1080/19443994.2013.780998