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- Title
The Thellungiella salsuginea Tonoplast Aquaporin TsTIP1;2 Functions in Protection Against Multiple Abiotic Stresses.
- Authors
Wang, Li-Li; Chen, An-Ping; Zhong, Nai-Qin; Liu, Ning; Wu, Xiao-Min; Wang, Fang; Yang, Chun-Lin; Romero, Michael F.; Xia, Gui-Xian
- Abstract
Examination of aquaporin (AQP) membrane channels in extremophile plants may increase our understanding of plant tolerance to high salt, drought or other conditions. Here, we cloned a tonoplast AQP gene (TsTIP1;2) from the halophyte Thellungiella salsuginea and characterized its biological functions. TsTIP1;2 transcripts accumulate to high levels in several organs, increasing in response to multiple external stimuli. Ectopic overexpression of TsTIP1;2 in Arabidopsis significantly increased plant tolerance to drought, salt and oxidative stresses. TsTIP1;2 had water channel activity when expressed in Xenopus oocytes. TsTIP1;2 was also able to conduct H2O2 molecules into yeast cells in response to oxidative stress. TsTIP1;2 was not permeable to Na+ in Xenopus oocytes, but it could facilitate the entry of Na+ ions into plant cell vacuoles by an indirect process under high-salinity conditions. Collectively, these data showed that TsTIP1;2 could mediate the conduction of both H2O and H2O2 across membranes, and may act as a multifunctional contributor to survival of T. salsuginea in highly stressful habitats.
- Subjects
TONOPLASTS; AQUAPORINS; MICROBIOLOGY of extreme environments; EFFECT of salts on plants; EFFECT of drought on plants; HALOPHYTES; ARABIDOPSIS
- Publication
Plant & Cell Physiology, 2014, Vol 55, Issue 1, p148
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/pcp/pct166