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- Title
Cysteamine and β-mercaptoethanol supplementation improves the in vitro embryo development in buffaloes (Bubalus bubalis).
- Authors
Singh, Vijay; Misra, A. K.; Kumar, Suresh; Barman, Champak
- Abstract
The objective of the present experiment was to investigate the effect of cysteamine and β-mercaptoethanol supplementation on in -vitro maturation, cleavage of oocytes and development of embryo in buffalo (Bubalus bubalis). Oocytes were aspirated from abattoir ovarian follicles of 3-10 mm diameter followed by maturation in the media in vitro containing cysteamine/β-mercaptoethanol (treatment) and without antioxidant (control). Matured oocytes were co-incubated with sperm (approx.1×106/ml) of Murrah bull in mSOF medium using heparin (10 μg/ml). After 22 h of oocyte-sperm incubation, fertilized oocytes were stripped of cumulus cells and cultured in mSOF medium for 8 days to study embryo development. The oocyte maturation rate improved significantly (P<0.05) following addition of 50 or 100 μM of cysteamine and 10, 50 and 100 μM of β- mercaptoethanol (ME), respectively as compared to control. The cleavage rate was found to be significantly (P<0.05) higher at 50 and 100 μM of cysteamine and at all concentrations of b-mercaptoethanol as compared to control and development of embryos to morula stage was significantly (P<0.05) improved with 50 μM cysteamine/ β-mercaptoethanol.
- Subjects
CYSTEAMINE; MERCAPTOETHANOL; CATTLE embryology; WATER buffalo; CLEAVAGE (Embryology); REPRODUCTION
- Publication
Indian Journal of Animal Research, 2016, Vol 50, Issue 3, p305
- ISSN
0367-6722
- Publication type
Article
- DOI
10.18805/ijar.9490