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- Title
An efficient process for obtaining prebiotic oligosaccharides derived from lactulose using isomerized and purified whey permeate.
- Authors
Sabater, Carlos; Olano, Agustín; Prodanov, Marin; Montilla, Antonia; Corzo, Nieves
- Abstract
BACKGROUND One of the most promising uses of whey permeate ( WP) is the synthesis of prebiotic oligosaccharides. Herein, commercial WP was submitted to chemical isomerization catalysed by sodium borate at an alkaline pH and subsequent purification using anion-exchange resins to remove boron. Subsequently, purified mixtures were used to synthesize prebiotic oligosaccharides using β-galactosidase from Bacillus circulans. RESULTS Isomerization of concentrated WP (200 g L−1 lactose) gave rise to levels of lactulose up to 155.5 g L−1 after 30 min of reaction (molar ratio of boron/lactose, 1/1; pH 12; 70 °C). Boron was removed from the isomerized WP ( IWP) using the combination of a strong acid ( IR-120, H+) and a weak base ( IRA-743) anion-exchange resins, reducing its level to <1 ppm, without loss of lactulose. During the transglycosylation reaction of purified IWP (lactose/lactulose ratio, 1/2.4) maximum content of prebiotic compounds was achieved, i.e. 690 g kg−1 WP after 3 h of reaction. CONCLUSION This study shows that combined chemical-enzymatic reactions together with the purification of IWP results in an efficient synthesis of prebiotic oligosaccharides. © 2017 Society of Chemical Industry
- Subjects
PREBIOTICS; WHEY processing; LACTULOSE; DAIRY processing; BACILLUS circulans
- Publication
Journal of the Science of Food & Agriculture, 2017, Vol 97, Issue 15, p5074
- ISSN
0022-5142
- Publication type
Article
- DOI
10.1002/jsfa.8384