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- Title
The Getting the Purification and the Characterization of Species' Immunoglobulin G (IgG) and Anti-immunoglobulin G Serum for Dog, Goat and Rabbit.
- Authors
Turcu, D.; Oporanu, Mariana; Grigorescu, P.; Condur, D.; Petruţ, T.; Simion, Vioteta; Călin, V.
- Abstract
The research aimed at preparing, purifying and characterizing the immunoglobulin 0, for dog, goat and rabbit, and the anti-IgG serum for the same species. The IgG species purification was done through precipitation, using ammonium sulfate solution and ion exchange chromatography (DEAE-cellulose). Checking the IgG purity was done by immunoelectrophoresis (IEF), for the corresponding anti-species serum, and by polyacrylamide gel electrophoresis in denaturant system (SDS-PAGE). Using IEF, we obtained a precipitation arch with cathodic migration, whereas using SDS-PAGE, we obtained a fraction with a molecular mass of 150 KDa. Dog and goat anti-IgG serum was prepared on rabbit, and the rabbit anti-IgG serum on goat, by IgG hyperimmunisation concealed in Freund adjuvant at a 1 to 1 ratio. Sensitivity and specificity testing of antisera was performed by immunoenzymatic test (ELISA). There were highlighted the high titers of antibodies to the dilution of. 1:500 for anti-IgG rabbit and goat sera, and 1:400 for anti-IgG dog serum. Specificity testing of dog and rabbit anti-IgG in the heterologous system showed results which are in the negative range. The DO values were high for goat anti-IgG serum, sheep IgG serum and sheep anti-IgG serum and goat anti-IgG serum, due to antigenic kinship between these species. The reagents prepared will be used in great specificity and sensitivity immunodiagnostic tests.
- Subjects
IMMUNOGLOBULIN G; LABORATORY animals; AMMONIUM sulfate; ION exchange (Chemistry); CHROMATOGRAPHIC analysis; ELECTROPHORESIS
- Publication
Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2010, Vol 67, Issue 1, p346
- ISSN
1843-5270
- Publication type
Article