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- Title
Evaluation of Anti-colitic Effect of Lactic Acid Bacteria in Mice by cDNA Microarray Analysis.
- Authors
Lee, Hoyong; Ahn, Young-Tae; Lee, Jung-Hee; Huh, Chul-Sung; Kim, Dong-Hyun
- Abstract
To evaluate the anti-colitic effect of lactic acid bacteria by cDNA microarray analysis, a lactic acid bacteria mixture (LM) consisting of Lactobacillus brevis HY7401, L. suntoryeus HY7801 and Bifidobacterium longum HY8004 was orally administered to dextran sulfate (DSS)-induced colitic mice and the expression profile of numerous genes was assessed. DSS treatment caused colitic outcomes such as inflammation and colon shortening. DSS also up-regulated the expression of inflammation-related genes: pro-inflammatory and chemotactic cytokines, including IL-1β, TNF-α, IL-6, CCL2, CCL4, CCL7, CCL24, CXCL1, CXCL2, CXCL5, CXCL9 and CXCL10, and their receptors CCR3 and CCR7, and other colitis-related genes such as COX-2, PAP, MMP family, S100a8, S100a9 and DEFA1. LM treatment inhibited the mRNA expression of inflammation-related and tissue remodeling genes induced by DSS as well as the colitic symptoms. LM inhibition for the DSS-induced expression of the representative inflammatory markers, IL-1β, TNF-α and COX-2, was supported by quantitative real-time polymerase chain reaction analysis. These findings suggest that LM ameliorates DSS-induced colitis by regulating inflammatory-related cytokines as well as tissue remodeling genes.
- Subjects
LACTIC acid bacteria; DNA microarrays; CYTOKINES; POLYMERASE chain reaction; MESSENGER RNA; CHEMOKINES
- Publication
Inflammation, 2009, Vol 32, Issue 6, p379
- ISSN
0360-3997
- Publication type
Article
- DOI
10.1007/s10753-009-9146-y