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- Title
Food preservative sorbic acid deregulates hepatic fatty acid metabolism.
- Authors
Chia-Hui Chen; Sin-Ni Ho; Po-An Hu; Yu Ru Kou; Tzong-Shyuan Lee
- Abstract
Sorbic acid (SA) is one of the most commonly used food preservatives worldwide. Despite SA having no hepato-toxicity at legal dosages, its effect on hepatic lipid metabolism is still unclear. We investigated the effect of SA on hepaticlipid metabolism and its mechanism of action in C57BL/6 mice. Daily treatment with SA (1 g/kg in diet) for 4 weeks didnot alter the body weight, organ weight, and blood lipids in mice. However, hepatic lipid accumulation, particularly thatof triglycerides, fatty acids, and glycerol, but not cholesteryl ester and free cholesterol, was increased with SA treatment.Mechanistically, SA decreased the expression of proteins related to de novo fatty acid lipogenesis, fatty acid internalization, and very low-density lipoprotein (VLDL) secretion-related pathways, including sterol regulatory element-binding proteins, acetyl-coA carboxylase, fatty acid synthase, liver fatty acid-binding protein, CD36, and apolipoproteinE. In contrast, SA increased the expression of diacylglycerol O-acyltransferase 2, the key enzyme for triacylglycerolsynthesis. Moreover, SA downregulated the protein expression of autophagy-related and β-oxidation-related pathways,the two major metabolic pathways for lipid metabolism, including LC-3, beclin-1, autophagy related protein 5 (ATG-5)and ATG-7, acyl-CoA synthetase long chain family member 1, carnitine palmitoyltransferase Iα, peroxisome pro-liferator-activated receptor α(PPARα), PPARγ, and PPARγ coactivator-1. Collectively, SA deregulates de novo lipo-genesis and fatty acid internalization, VLDL secretion, autophagy, and β-oxidation in the liver, leading to impaired lipidclearance and ultimately, resulting in lipid accumulation in the liver.
- Subjects
LIPID metabolism; AUTOPHAGY; ANIMAL experimentation; APOLIPOPROTEINS; FATTY acids; FOOD preservatives; GENE expression; GLYCERIN; LIPIDS; LIVER; LOW density lipoproteins; METABOLISM; MICE; PROTEINS; TRIGLYCERIDES; UNSATURATED fatty acids
- Publication
Journal of Food & Drug Analysis, 2020, Vol 28, Issue 2, p12
- ISSN
1021-9498
- Publication type
Article
- DOI
10.38212/2224-6614.1055