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- Title
Differential phosphorylation of SNAP-25 in vivo by protein kinase C and protein kinase A
- Authors
Hepp, Régine; Cabaniols, Jean-Pierre; Roche, Paul A.
- Abstract
SNAP-25 is a key protein required for the fusion of synaptic vesicles with the plasma membrane during exocytosis. This study establishes that SNAP-25 is differentially phosphorylated by protein kinase C and protein kinase A in neuroendocrine PC12 cells. Using phosphopeptide mapping and site-directed mutagenesis we identified both Thr138 and Ser187 as the targets of SNAP-25 phosphorylation by protein kinase C and Thr138 as the exclusive site of SNAP-25 phosphorylation by protein kinase A in vivo. Finally, despite published data to the contrary, we demonstrate that stimulation of regulated exocytosis under physiological conditions is independent of a measurable increase in SNAP-25 phosphorylation in PC12 cells.
- Subjects
EXOCYTOSIS; PROTEIN kinase C
- Publication
FEBS Letters, 2002, Vol 532, Issue 1/2, p52
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03629-3