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- Title
Stress-induced premature senescence in BJ and hTERT-BJ1 human foreskin fibroblasts
- Authors
de Magalhães, João Pedro; Chainiaux, Florence; Remacle, José; Toussaint, Olivier
- Abstract
To test the involvement of the telomeres in the senescent phenotype, we used telomerase-immortalized human foreskin fibroblasts (hTERT-BJ1). We exposed hTERT-BJ1 and parental BJ cells to either UVB or H2O2 subcytotoxic stress(es). Both cell lines developed biomarkers of replicative senescence: loss of replicative potential, increase in senescence-associated β-galactosidase activity, typical senescence-like morphology, overexpression of p21WAF-1 and p16INK-4a, and decreased level of the hyperphosphorylated form of pRb. Telomere shortening was slightly higher under stress for both BJ and hTERT-BJ1 but still much lower than that reported for other cell lines. We conclude that pathways alternative to telomere shortening must cause the appearance of the senescence phenotype.
- Subjects
TELOMERES; PEROXIDES; FIBROBLASTS; PHYSIOLOGICAL stress
- Publication
FEBS Letters, 2002, Vol 523, Issue 1-3, p157
- ISSN
0014-5793
- Publication type
Article