We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Role of intracellular loops of cannabinoid CB<sub>1</sub> receptor in functional interaction with G<sub>α16</sub>
- Authors
Ho, Begonia Y.; Current, Lori; Drewett, James G.
- Abstract
The cannabinoid CB1 but not the CB2 receptor was demonstrated to couple via Gα16 to activate phospholipase C after co-expression in COS7 cells. Chimeric CB1/CB2 receptors were used as a model to study receptor–Gα16 interaction. Sequences of the second and third intracellular loops and the carboxy-terminus were substituted from the CB1 into the CB2 receptor. Only the triple mutant with all three regions replaced activated phospholipase C to a similar extent as the CB1 receptor, suggesting that all three intracellular regions are required for interacting with Gα16. Several sub-domains within the third intracellular loop were identified for receptor–Gα16 interaction.
- Subjects
CANNABINOIDS; PHOSPHOLIPASES
- Publication
FEBS Letters, 2002, Vol 522, Issue 1-3, p130
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)02917-4