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- Title
RAPID AND SENSITIVE DETECTION OF LAWSONIA INTRACELLULARIS IN PIGS BY REAL-TIME LOOP-MEDIATED ISOTHERMAL AMPLIFICATION.
- Authors
Byung-Yong, PARK; Kwan-Seob, SHIM; Won-Il, KIM; Md Mukter, HOSSAIN; Bumseok, KIM; KWON Jungkee; Choi-Kyu, PARK; CHO Sung-Jin; JO Inho; CHO Ho-Seong
- Abstract
A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured L. intracellularis and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in L. intracellularis was 1.0 × 10° L. intracellularis. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of L. intracellularis in porcine fecal samples.
- Subjects
LAWSONIA; INTESTINAL diseases; SWINE diseases; UBIQUINONES; VITAMIN K2; METHYLTRANSFERASES
- Publication
Acta Veterinaria, 2015, Vol 65, Issue 1, p20
- ISSN
0567-8315
- Publication type
Article
- DOI
10.1515/acve-2015-0002