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- Title
Bovine Serum Albumin Protein-Based Liquid Crystal Biosensors for Optical Detection of Toxic Heavy Metals in Water.
- Authors
Amin, Noor ul; Siddiqi, Humaira Masood; Kun Lin, Yang; Hussain, Zakir; Majeed, Nasir
- Abstract
A new methodology involving the use of Bovine Serum Albumin (BSA) as a probe and liquid crystal (LC) as a signal reporter for the detection of heavy metal ions in water at neutral pH was developed. BSA acted as a multi-dentate ligand for the detection of multiple metal ions. The LC sensor was fabricated by immobilizing 3 µg mL−1 BSA solution on dimethyloctadecyl-[3-(trimethoxysilyl)propyl]ammonium chloride (DMOAP)-coated glass slides. In the absence of heavy metal ions, a dark optical image was observed, while in their presence, a dark optical image turned to bright. The optical response was characterized by using a polarized optical microscope (POM). The BSA based LC sensor selectively detected toxic metal ions as compared to s block metal ions and ammonium ions in water. Moreover, the limit of detection was found to be very low (i.e., 1 nM) for the developed new biosensor in comparison to reported biosensors.
- Subjects
DEUTERIUM oxide; LIQUID crystals; HEAVY metals; METAL ions; PHYTOCHELATINS; POLYMER liquid crystals; AMMONIUM ions; SERUM albumin
- Publication
Sensors (14248220), 2020, Vol 20, Issue 1, p298
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s20010298