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- Title
Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans.
- Authors
Su, Han; Li, Shaopei; Terebiznik, Mauricio; Guyard, Cyril; Kerman, Kagan
- Abstract
The adhesin Legionella collagen-like (Lcl) protein can bind to extracellular matrix components and mediate the binding of <italic>Legionella pneumophila</italic> to host cells. In this study, electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR)-based biosensors were employed to characterize these interactions between glycosaminoglycans (GAGs) and the adhesin Lcl protein. Fucoidan displayed a high affinity (KD 18 nM) for Lcl protein. Chondroitin sulfate A and dermatan sulfate differ in the position of a carboxyl group replacing D-glucuronate with D-iduronate. Our results indicated that the presence of D-iduronate in dermatan sulfate strongly hindered its interaction with Lcl. These biophysical studies provided valuable information in our understanding of adhesin-ligand interactions related to <italic>Legionella pneumophila</italic> infections.
- Subjects
BIOSENSORS; LEGIONELLA pneumophila; GLYCOSAMINOGLYCANS; EXTRACELLULAR matrix proteins; ARTIFICIAL neural networks
- Publication
Sensors (14248220), 2018, Vol 18, Issue 8, p2668
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s18082668