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- Title
UBP1, a novel hnRNP-like protein that functions at multiple steps of higher plant nuclear pre-mRNA maturation.
- Authors
Lambermon, Mark H. L.; Simpson, Gordon G.; Kirk, Dominika A. Wieczorek; Hemmings-Mieszczak, Maja; Klahre, Ulrich; Filipowicz, Witold
- Abstract
Efficient splicing of higher plant pre-mRNAs depends on AU- or U-rich sequences in introns. Moreover, AU-rich sequences present in 3′-untranslated regions (3′-UTRs) may play a role in 3′ end processing of plant mRNAs. Here, we describe the cloning and characterization of a Nicotiana plumbaginifolia nuclear protein that can he cross-linked to U-rich intron and 3′-UTR sequences in vitro, and associates with nuclear poly(A)+ RNA in vivo. The protein, UBP1, strongly enhances the splicing of otherwise inefficiently processed introns when overexpressed in protoplasts. It also increases the accumulation of reporter mRNAs that contain suboptimal introns or are intronless. The enhanced accumulation is apparently due to UBP1 interacting with the 3′-UTR and protecting mRNA from exonucleolytic degradation. The effect on mRNA accumulation hut not on mRNA splicing was found to be promoter specific. The fact that these effects of UBP1 can be separated suggests that they represent two independent activities. The properties of UBP1 indicate that it is an hnRNP protein that functions at multiple steps to facilitate the nuclear maturation of plant pre-mRNAs.
- Subjects
MESSENGER RNA; INTRONS; PLANT genetics; NICOTIANA; CLONING; PROTOPLASTS; RNA splicing
- Publication
EMBO Journal, 2000, Vol 19, Issue 7, p1638
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/19.7.1638