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- Title
Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria.
- Authors
Rusché, Laura N.; Cruz-Reyes, Jorge; Piller, Kenneth J.; Sollner-Webb, Barbara
- Abstract
Kinetoplastid mitochondrial RNA editing, the insertion and deletion of U residues, is catalyzed by sequential cleavage, U addition or removal, and ligation reactions and is directed by complementary guide RNAs. We have purified a ′20S enzymatic complex from Trypanosoma brucei mitochondria that catalyzes a complete editing reaction in vitro. This complex possesses all four activities predicted to catalyze RNA editing: gRNA-directed endonuclease, terminal uridylyl transferase, 3′ U-specific exonuclease, and RNA ligase. However, it does not contain other putative editing complex components: gRNA-independent endonuclease, RNA helicase, endogenous gRNAs or pre-mRNAs, or a 25 kDa gRNA-binding protein. The complex is composed of eight major polypeptides, three of which represent RNA ligase. These findings identify polypeptides representing catalytic editing factors, reveal the nature of this ∼20S editing complex, and suggest a new model of editosome assembly.
- Subjects
RNA editing; TRYPANOSOMA brucei; MITOCHONDRIA; MESSENGER RNA; ENDONUCLEASES; CARRIER proteins
- Publication
EMBO Journal, 1997, Vol 16, Issue 13, p4069
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/16.13.4069