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- Title
Adipose Triglyceride Lipase (ATGL) Has a Role in Muscle Lipid Oxidation and Is Upregulated by Pioglitazone in Adipose Tissue of Humans.
- Authors
Yao-Borengasser, Aiwei; Varma, Vijayalakshmi; Bodles, Angela M.; Rasouli, Neda; Phanavanh, Bounleut; Ranganathan, Gourj; Mcgehee, Robert E.; Kern, Philip A.
- Abstract
Adipose triglyceride lipase (ATGL) has been identified as an important mediator of adipocyte lipolysis and energy homeostasis. ATGL deficient mice had increased adipose mass and triglyceride accumulation in cardiac muscle. We studied the expression of ATGL in human fat and muscle, and examined correlations with muscle lipid oxidation. ATGL was expressed in mature adipocytes at a level 10-fold higher than that in the adipose tissue stromal fraction or muscle. We measured ATGL mRNA levels in adipose tissue and muscle of 86 nondiabetic subjects covering a wide range of BMI (19-55 Kg/m²) and S[sub 1] (1.0226.77 x 10[sup -5] x min[sup -1]/pM), with either normal glucose tolerance (NGT) or impaired glucose tolerance (IGT). ATGL expression in adipose tissue or muscle showed no correlation with either BMI or St. In muscle, ATGL mRNA associated strongly with genes involved with lipid oxidation, including CPT1 mRNA (r=0.82, p<0.0001), AdipoR1 mRNA (r=0.71, p<0.0001), and AdipoR2 mRNA (r=0.74, p<0.0001), however there was no association with intramyocellular lipid. Thirty two IGT subjects were randomized to receive either pioglitazone (PIO) or metformin (MET) for 10 weeks, and we examined the effects of these drugs on ATGL expression. PIO, but not MET, resulted in a 31% increase of adipose ATGL expression (p<0.03), with no change in muscle. ATGL in muscle is associated with lipid oxidation. These data tend to support rodent studies which suggest that muscle ATGL may be involved with the release of FFA from lipid storage for oxidation. However, adipose ATGL expression was not associated with obesity or insulin resistance, and was increased following PIO treatment, suggesting an important role in the balance of adipose lipid metabolism.
- Subjects
ADIPOSE tissues; TRIGLYCERIDES; LIPASES; FAT cells; LIPOLYSIS; HOMEOSTASIS; MYOCARDIUM; LIPID metabolism
- Publication
Diabetes, 2007, Vol 56, pA357
- ISSN
0012-1797
- Publication type
Article