We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Salt Dependence of DNA Binding Activity of Human Transcription Factor Dlx3.
- Authors
Jin, Ho-Seong; Son, Juyeon; Seo, Yeo-Jin; Choi, Seo-Ree; Ahn, Hye-Bin; Go, Youyeon; Lim, Juhee; Oh, Kwang-Im; Ryu, Kyoung-Seok; Lee, Joon-Hwa
- Abstract
Distal-less 3 (Dlx3) is a homeobox-containing transcription factor and plays a crucial role in the development and differentiation process. Human Dlx3 consists of two transactivation domains and a homeobox domain (HD) that selectively binds to the consensus site (5′-TAATT-3′) of the DNA duplex. Here, we performed chemical shift perturbation experiments on Dlx3-HD in a complex with a 10-base-paired (10-bp) DNA duplex under various salt conditions. We also acquired the imino proton spectra of the 10-bp DNA to monitor the changes in base-pair stabilities during titration with Dlx3-HD. Our study demonstrates that Dlx3-HD selectively recognizes its consensus DNA sequences through the α3 helix and L1 loop regions with a unique dynamic feature. The dynamic properties of the binding of Dlx3-HD to its consensus DNA sequence can be modulated by varying the salt concentrations. Our study suggested that this unique structural and dynamic feature of Dlx3-HD plays an important role in target DNA recognition, which might be associated with tricho-dento-osseous syndrome.
- Subjects
TRANSCRIPTION factors; DNA; NUCLEOTIDE sequence; SALT; DNA sequencing; DNA-protein interactions
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 16, p9497
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23169497