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- Title
IKBKB siRNA-Encapsulated Poly (Lactic- co -Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation.
- Authors
Lee, Seounghun; Shin, Hyo-Jung; Noh, Chan; Kim, Song-I; Ko, Young-Kwon; Lee, Sun-Yeul; Lim, Chaeseong; Hong, Boohwi; Yang, Sin-Young; Kim, Dong-Woon; Lee, Won-Hyung; Kim, Yoon-Hee
- Abstract
Activation of nuclear factor-kappa B (NF-κB) in microglia plays a decisive role in the progress of neuropathic pain, and the inhibitor of kappa B (IκB) is a protein that blocks the activation of NF-κB and is degraded by the inhibitor of NF-κB kinase subunit beta (IKBKB). The role of IKBKB is to break down IκB, which blocks the activity of NF-kB. Therefore, it prevents the activity of NK-kB. This study investigated whether neuropathic pain can be reduced in spinal nerve ligation (SNL) rats by reducing the activity of microglia by delivering IKBKB small interfering RNA (siRNA)-encapsulated poly (lactic-co-glycolic acid) (PLGA) nanoparticles. PLGA nanoparticles, as a carrier for the delivery of IKBKB genes silencer, were used because they have shown potential to enhance microglial targeting. SNL rats were injected with IKBKB siRNA-encapsulated PLGA nanoparticles intrathecally for behavioral tests on pain response. IKBKB siRNA was delivered for suppressing the expression of IKBKB. In rats injected with IKBKB siRNA-encapsulated PLGA nanoparticles, allodynia caused by mechanical stimulation was reduced, and the secretion of pro-inflammatory mediators due to NF-κB was reduced. Delivering IKBKB siRNA through PLGA nanoparticles can effectively control the inflammatory response and is worth studying as a treatment for neuropathic pain.
- Subjects
GLYCOLIC acid; NEURALGIA; MICROGLIA; NF-kappa B; SMALL interfering RNA; NANOPARTICLES
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 11, p5657
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms22115657