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- Title
The Complete Structure of the Core Oligosaccharide from Edwardsiella tarda EIB 202 Lipopolysaccharide.
- Authors
Kaszowska, Marta; de Mendoza-Barberá, Elena; Maciejewska, Anna; Merino, Susana; Lugowski, Czeslaw; Tomás, Juan M.
- Abstract
he chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharide of pathogenic Edwardsiella tarda strain EIB 202 were studied for the first time. The complete gene assignment for all LPS core biosynthesis gene functions was acquired. The complete structure of core oligosaccharide was investigated by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry MSn, and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. The following structure of the undecasaccharide was established:β-d-Galp1↓3 β-d-Glcp 1↓α-D-GlcpNAcGly-(1→4)-α-D-GalpA-(1→3)-α-L,D-Hepp-(1↑)-α-L,D-Hepp-(1α)-α-Kdo α-D-GlcpNAcGly-(1→4)-α-D-GalpA-(1→7)-α-L,D-Hepp1↑ β-D-Glcp 1↑4 The heterogeneous appearance of the core oligosaccharide structure was due to the partial lack of β-d-Galp and the replacement of α-d-GlcpNAcGly by α-d-GlcpNGly. The glycine location was identified by mass spectrometry.
- Subjects
OLIGOSACCHARIDES; EDWARDSIELLA tarda; LIPOPOLYSACCHARIDES; NUCLEAR magnetic resonance spectroscopy; ELECTROSPRAY ionization mass spectrometry
- Publication
International Journal of Molecular Sciences, 2017, Vol 18, Issue 6, p1163
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms18061163