We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The Interaction of CuS and Halothiobacillus HT1 Biofilm in Microscale Using Synchrotron Radiation-Based Techniques.
- Authors
Huirong Lin; Guangcun Chen; Shenhai Zhu; Yingxu Chen; Dongliang Chen; Wei Xu; Xiaohan Yu; Jiyan Shi
- Abstract
In order to investigate the microbe-mineral interaction in the micro scale, spatial distribution and speciation of Cu and S in Halothiobacillus HT1 biofilm formed on a CuS surface was examined using synchrotron-based X-ray techniques. Confocal laser scanning microscope (CLSM) results indicated that Halothiobacillus HT1 biofilm formation gave rise to distinct chemical and redox gradients, leading to diverse niches in the biofilm. Live cells were distributed at the air-biofilm and membrane-biofilm interface. CuS was oxidized by Halothiobacillus HT1 biofilm, and copper penetrated into the biofilm. Sulfide was oxidized to cysteine (77.3%), sulfite (3.8%) and sulfonate (18.9%). Cu-cysteine-like species were involved in the copper homeostasis. These results significantly improve our understanding of the interfacial properties of the biofilm-mineral interface.
- Subjects
BIOFILMS; LASER microscopy; ECOLOGICAL niche; COPPER sulfide; CYSTEINE; SULFITES; SULFONATES
- Publication
International Journal of Molecular Sciences, 2013, Vol 14, Issue 6, p11113
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms140611113