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- Title
Hemagglutinin virus-like particles incorporated with membrane-bound cytokine adjuvants provide protection against homologous and heterologous influenza virus challenge in aged mice.
- Authors
Park, Bo Ryoung; Bommireddy, Ramireddy; Chung, David Hyunjung; Kim, Ki-Hye; Subbiah, Jeeva; Jung, Yu-Jin; Bhatnagar, Noopur; Pack, Christopher D.; Ramachandiran, Sampath; Reddy, Shaker J.C.; Selvaraj, Periasamy; Kang, Sang-Moo
- Abstract
Background: Current influenza vaccines deliver satisfactory results in young people but are less effective in the elderly. Development of vaccines for an ever-increasing aging population has been an arduous challenge due to immunosenescence that impairs the immune response in the aged, both quantitatively and qualitatively. Results: To potentially enhance vaccine efficacy in the elderly, we investigated the immunogenicity and cross-protection of influenza hemagglutinin virus-like particles (HA-VLP) incorporated with glycosylphosphatidylinositol (GPI)-anchored cytokine-adjuvants (GPI-GM-CSF and GPI-IL-12) via protein transfer in aged mice. Lung viral replication against homologous and heterologous influenza viruses was significantly reduced in aged mice after vaccination with cytokine incorporated VLPs (HA-VLP-Cyt) in comparison to HA-VLP alone. Enhanced IFN-γ+CD4+ and IFN-γ+CD8+ T cell responses were also observed in aged mice immunized with HA-VLP-Cyt when compared to HA-VLP alone. Conclusions: Cytokine-adjuvanted influenza HA-VLP vaccine induced enhanced protective response against homologous influenza A virus infection in aged mice. Influenza HA-VLP vaccine with GPI-cytokines also induced enhanced T cell responses correlating with better protection against heterologous infection in the absence of neutralizing antibodies. The results suggest that a vaccination strategy using cytokine-adjuvanted influenza HA-VLPs could be used to enhance protection against influenza A virus in the elderly.
- Subjects
VIRUS-like particles; INFLUENZA A virus; INFLUENZA viruses; YOUNG adults; HEMAGGLUTININ
- Publication
Immunity & Ageing, 2023, Vol 20, Issue 1, p1
- ISSN
1742-4933
- Publication type
Article
- DOI
10.1186/s12979-023-00344-w