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- Title
Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair.
- Authors
Singh, Kuldeep; Miller, Myrna M.; Kohrt, Laura J.; Scherba, Gail; Garrett, Edgar F.; Fredrickson, Richard L.
- Abstract
The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1~3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30~100) and whole blood. All 23 animals were positive for the virus by qRT-PCR performed on the whole blood and when samples of more than 30 hairs were assayed. Additionally, qRT-PCR was performed on groups of 10 and 20 hairs harvested from 7 out of 23 immunohistochemical staining-positive calves. When groups of 20 and 10 hairs were tested, 6 and 4 animals, respectively, were positive for the virus.
- Subjects
BOVINE viral diarrhea diagnosis; BOVINE viral diarrhea virus; NONINVASIVE diagnostic tests; REVERSE transcriptase polymerase chain reaction; BLOOD testing; HAIR; IMMUNOHISTOCHEMISTRY
- Publication
Journal of Veterinary Science, 2011, Vol 12, Issue 3, p295
- ISSN
1229-845X
- Publication type
Article
- DOI
10.4142/jvs.2011.12.3.295