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- Title
Vacuolar targeting of aldehyde dehydrogenase 6 tagging with signal peptide of proteinase A.
- Authors
Park, Dong J.; Choi, Wooil; Bang, Seung H.; Kim, Sang Y.; Wee, Ji‐Hyang; Kim, Yang‐Hoon; Min, Jiho
- Abstract
Vacuoles are useful materials with antimicrobial and anticancerous properties. Vacuolar proteins can discompose macromolecules from the outside of yeast cells. The objective of this study was to determine the function of a protein transported into a vacuole. Specifically, cytosolic protein aldehyde dehydrogenase 6 (ALD6) was used for the delivery to the vacuole. To transport cytosolic protein to the vacuole in this study, a transfer vector including a signal peptide sequence isolated from vacuolar protein proteinase A was designed. A signal peptide is an amino acid sequence in front of the transported protein. Signal peptides have various delivery pathways according to the kind of signal sequence they contain. They play important roles in transporting proteins to organelles, in cellular mechanisms, and the transfer of protein outside and inside eukaryotes. Thus, we focused on the design of a transfer vector containing a signal peptide sequence isolated from the DNA sequence of proteinase A (PEP4). In addition, this study evaluated the expression level of cytosolic ALD6 after being transported into the yeast vacuole. Our results showed that the developed transfer vector was useful for delivering proteins to vacuole by using signal peptide sequence. Therefore, this transfer vector might be used as a tool to deliver target proteins to organelles of interest in eukaryotes.
- Subjects
ALDEHYDE dehydrogenase; CARRIER proteins; AMINO acid sequence; SERINE proteinases; SIGNAL peptides; MACROMOLECULES; PROTEINASES
- Publication
Journal of Basic Microbiology, 2020, Vol 60, Issue 4, p341
- ISSN
0233-111X
- Publication type
Article
- DOI
10.1002/jobm.201900579