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- Title
Characterization of the divergent eosinophil ribonuclease, mEar 6, and its expression in response to Schistosoma mansoni infection in vivo.
- Authors
Nitto, T; Dyer, K D; Mejia, R A; Byström, J; Wynn, T A; Rosenberg, H F
- Abstract
The eosinophil-associated ribonucleases (Ears) are rapidly evolving proteins found in multigene clusters that are unique to each rodent species. Of the 15 independent genes in the Mus musculus cluster, only mEars 1 and 2 are expressed at significant levels at homeostasis. Here we characterize the expression of mEar 6 in the liver and spleen in mice in response to infection with the helminthic parasite, Schistosoma mansoni. Interestingly, expression of mEar 6 is not directly related to the elevated levels of serum IL-5 or tissue eosinophilia characteristic of this disease, as no mEar 6 transcripts were detected in the liver or the spleen from uninfected IL-5-transgenic mice. The coding sequence of mEar 6 has diverged under positive selection pressure (Ka/Ks>1.0) and has a unique unpaired cysteine near the carboxy-terminus of the protein. The high catalytic efficiency of recombinant mEar 6 (kcat/Km=0.9×106/M/s) is similar to that of the cluster's closest human ortholog, eosinophil-derived neurotoxin (EDN/RNase 2). In summary, we have identified mEar 6 as one of only two RNase A superfamily ribonucleases known to be expressed specifically in response to pathophysiologic stress in vivo.Genes and Immunity (2004) 5, 668-674. doi:10.1038/sj.gene.6364143 Published online 4 November 2004
- Subjects
SCHISTOSOMA mansoni; RIBONUCLEASES; EOSINOPHILS; INTERLEUKIN-5; IMMUNITY
- Publication
Genes & Immunity, 2004, Vol 5, Issue 8, p668
- ISSN
1466-4879
- Publication type
Article
- DOI
10.1038/sj.gene.6364143