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- Title
Molecular Characterization and Phylogenetic Analysis of Rhizoctonia solani Kuhn. Infecting Tomato.
- Authors
NANDAN, M.; KUMAR, H. D. VINAY; HIREMATH, SHRIDHAR; MANTESH, M.; BASHA, C. R. JAHIR; REDDY, C. N. LAKSHMINARAYANA
- Abstract
Tomato is one of the important vegetable crops grown across various parts of the world. It suffers from various diseases among them, dry root rot caused by soil-borne pathogen Rhizoctonia solani Kuhn. is one of the major threat for tomato production. In this context, tomato plants suffering from dry root rot disease were collected, processed, pure culture of Rhizoctonia sp. was isolated on PDA media and designated as Rhizoctonia RSK isolate. The pure culture of isolated fungus was studied for its morphological characters through microscopic examination where, right angle branching of mycelium and sclerotial bodies formation typical to genus, Rhizoctonia were observed. Mass multiplied Rhizoctonia RSK isolate inoculum was inoculated to thirty days old tomato seedlings (Variety: Arka Vikas) which produced symptoms viz., yellowing of older leaves, necrotised lesion and rotting at the collar and root region similar to the symptoms observed for dry root on tomato in the field, confirming its pathogenicity. Further, to confirm the species level, genomic DNA was isolated from the Rhizoctonia RSK isolate, internal transcribed spacer (ITS) region was amplified using ITS1 and ITS4 primers and the amplicon was sequenced. ITS sequence and phylogenetic analysis revealed that the Rhizoctonia RSK isolate infecting tomato in the current study is Rhizoctonia solani, which shared maximum nucleotide identity with Ceratobasidium sp.
- Subjects
TOMATO farming; VEGETABLE farming; TOMATO diseases &; pests; SOILBORNE plant diseases; AGRICULTURAL productivity
- Publication
Mysore Journal of Agricultural Sciences, 2021, Vol 55, Issue 2, p45
- ISSN
0047-8539
- Publication type
Article