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- Title
Na<sup>+</sup>/H<sup>+</sup> exchanger 1 inhibition contributes to K562 leukaemic cell differentiation.
- Authors
Jin, Weina; Li, Qinghua; Wang, Jian; Chang, Guoqiang; Lin, Yani; Li, Huawen; Wang, Lihong; Gao, Wei; Pang, Tianxiang
- Abstract
The effect of hypoxia on the differentiation of chronic myeloid leukaemic K562 cells were studied, as was the role of the NHE1 (Na+/H+ exchanger 1). Hypoxia induced differentiation of K562 cells as seen by modifications in their morphological features, up-regulation of C/EBPα (CCAAT/enhancer-binding protein α), and marked IL-8 (interleukin-8) release. Inhibition of NHE1 under hypoxia additionally enhanced the level of C/EBPα and further promoted leukaemic cells differentiation. Pharmacological inhibition of p38 MAPK (mitogen-activated protein kinase) also significantly suppressed C/EBPα expression under hypoxia conditions after NHE1 inhibition. These results indicate the enhancement of hypoxia-induced K562 differentiation by NHE1 inhibition, which may be due to up-regulation of C/EBPα via p38 MAPK signalling pathway, which suggests a possible therapeutic target of NHE1 under hypoxia microenvironment in the treatment of leukaemic diseases.
- Subjects
SODIUM-hydrogen antiporter; LEUKEMIA; CANCER cell differentiation; HYPOXEMIA; CHRONIC myeloid leukemia; CARRIER proteins
- Publication
Cell Biology International, 2012, Vol 36, Issue 8, p739
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1042/CBI20100919