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- Title
设施番茄病毒病病原鉴定.
- Authors
孙晓军; 周婷婷; 玉山江·麦麦提; 何伟; 罗文芳; 许建军; 阿布都赛买提·吐尔送
- Abstract
【Objective】 To find out the virus species that harm the facility tomato in Luopu, Xinjiang, and provide the basis for scientific prevention and treatment of tomato virus disease in this area. 【Methods】 In the autumn of 2019, 8 tomato samples with suspected symptoms of virus disease in the protected vegetable producing area in Luopu were collected and the reported tomato viruses were detected by reverse transciption-PCR (RT-PCR) using specific primers of 7 main viruses. The CP gene of the positive sample ToCV was cloned and later sequenced. Meanwhile, homology, geographic origin and phylogeny analysis were conducted. 【Results】 The results showed that STV, TYLCV and ToCV were detected in 8 samples, and the detection rates were 100%, 100% and 87.5%, respectively. TMV, CMV, ToMV and ToMMV were not detected. The 774 bp sequences of CP genes isolated in Luopu had high homology with 51 ToCV CP genes reported, and the nucleotide sequence of the CP gene of ToCV isolate from Xinjinag shared high identity (up to 95%) with those of the ToCV isolates from China, Japan, South Korea, Greece, Turkey and Brazil. 【Conclusion】 The results showed that the etiolation of tomato plants collected from Loupu county were jointly infected by two or more viruses including STV, TYLCV and ToCV. It is the first time report that ToCV spread to Hotan, Xinjiang and infected tomatoes there.
- Publication
Xinjiang Agricultural Sciences, 2021, Vol 58, Issue 1, p99
- ISSN
1001-4330
- Publication type
Article
- DOI
10.6048/j.issn.1001-4330.2021.01.012