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- Title
RESEARCHES REGARDING PRODUCTION OF IN VITRO MICROTUBERS >10 mm ON POTATO.
- Authors
Chiru, Nicoleta; Nistor, Andreea; Karacsonyi, Diana; Chiru, Ruxandra Nicoleta
- Abstract
A technique for production of in vitro microtubers >10 mm was developed for some potato varieties (Solanum tuberosum L.). Uninodal cuttings from potato microplants propagated in vitro were utilized as initial ex-plants. These were placed in culture vessel containing medium with nutritional solutions specific for each phase of tuberisation process (growing, inducing and tuberisation). The plant material used, came from three Romanian potato cultivars (Roclas, Christian and Tampa), using into three variants of working: 15 and 25 uninodal cuttings/ vessel and five plantlets / vessel placed horizontally. For each stage of development (growth, inducing), cultures were kept under controlled conditions of light and temperature (16 hours of light/8 hours of dark, t0 20 ± 1°C/day and 18 ± 1°C/night). The cultures, over which to induce microtuberization, was added tuberization liquid medium, were transferred to the climate room at darkness, at a temperature of about 14 to 16°C. After roughly 6-7 weeks were obtained microtubers, improved on both in terms of number and size (> 10 mm) and also of weight (>950g).
- Subjects
PLANT growing media; PLANT micropropagation; TEMPERATURE of plants; POTATOES; CULTIVARS; SEED potatoes
- Publication
Annals of the Romanian Society for Cell Biology, 2010, Vol 15, Issue 2, p73
- ISSN
2067-3019
- Publication type
Article