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- Title
猪CAST基因多态性与生物信息学分析.
- Authors
许金根; 靳二辉; 王重龙; 顾有方; 李庆岗
- Abstract
The aim of this study was to detect the CAST gene polymorphism of Berkshire pig, Huoshou black pig and their hybrid F1 generation, and to analyze the effect of base variation on the mRNA secondary structure and CAST type Ⅲ protein by bioinformatics. The results showed that there were AA, AC and CC genotypes in Berkshire pig, Huoshou black pig and Berkshire × Huoshou black pig, and the frequency of allele A was higher than that of allele C. The mutation was in Hardy-Weinberg equilibrium in all three pig populations (P>0.05), and with moderate polymorphism (0.25<PIC<0.5). Bioinformatics prediction showed that c. 1980A>C slightly changed the secondary structure of porcine CAST gene mRNA sequence, and increased the minimum free energy. The p. Arg660Ser mutation resulted in a slight change in some physicochemical properties of the porcine CAST type Ⅲ protein, including molecular formula, molecular weight, isoelectric point, instability index, grand average of hydropathicity, positively charged residues (Arg and Lys) number. The secondary structure of porcine CAST type Ⅲ protein was not changed by the missense mutation, and the proportion of random coil and α-helix were higher before and after mutation. The p. Arg660Ser mutation was not located in the conserved domain of the porcine CAST type Ⅲ protein, but could lead to a decrease in the number of protein kinases that catalyzed the phosphorylation of adjacent p. 663Ser. In summary, the c.1980A>C mutation might have a certain impact on the transcription efficiency and function of the pig CAST gene.
- Publication
Acta Agriculturae Zhejiangensis, 2022, Vol 34, Issue 1, p17
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2022.01.03