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- Title
杜仲miR172基因家族的生物信息学分析与功能预测.
- Authors
叶靖; 杨元玲; 史庆秋; 吴龙飞; 宋国涛
- Abstract
To understand the evolutionary characteristics of the miR172 family members and their role in the growth and development of Eucommia ulmoides, the precursor and mature sequences of miR172 family were collected from the high-throughput sequencing results (accession number:SRP216820) and published literature related to E. ulmoides. In addition, sequence alignment, WebLogo conservation analysis, phylogenetic tree construction, precursor stem-loop structure prediction, promoter structure analysis, target gene prediction and functional prediction were performed. The E. ulmoides miR172 family contains 8 precursor sequences and 8 mature sequences, and all precursor sequences can form a stable stem-loop structure. Sequence alignment and WebLogo analysis showed that 7 mature sequences (except eu-miR172d-5p) were highly conservative, however the precursor sequences were less conservative. Evolutionary analysis showed that the mature sequences of this family were highly conservative, but the precursor sequences were less conservative. Promoter analysis showed that the E. ulmoides miR172 family promoters contained abundant photoreaction components and hormone response elements and stress response elements, indicating that they may regulate the process of flower organ formation and stress response. Target gene prediction showed that there were many target genes for miR172 family of E. ulmoides, and they were involved in E. ulmoides growth and development and adversity stress, but the miR172 family members mainly regulated the expression of AP2 genes. The E. ulmoides miR172 family members were both conservative and differentiated during evolution, and they play an important regulatory roles in the growth and development of E. ulmoides by regulating AP2 expression.
- Publication
Acta Agriculturae Zhejiangensis, 2022, Vol 34, Issue 1, p70
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2022.01.09