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- Title
Study on the Production of Pentachloronitrobenzene Monoclonal Antibody and Its ELISA Kit for Rapid Detection.
- Authors
Yulua MA; Kuo ZHANG; Jianxiong ZHANG; Fangfang JIA; Fangyang HE; Yanan CUI; Mingyang LI; Yuping WAN
- Abstract
[Objectives] This study was conducted to develop an enzyme-linked immunoassay kit that can detect the residual amount of pentachloronitrobenzene in Penaeus vannamei. [Methods] This study was conducted to develop an enzyme-linked immunoassay kit that can detect the residual amount of pentachloronitrobenzene in P. vannamei. [Results] The standard curve range of the kit was 0-8.1 μg/L; the detection limit for P. vannamei was 0.912 μg/kg; the recovery was 80.6%-103.5%; and the relative standard deviation range within batches was 5. 3% - 10. 1%, and the relative standard deviation range between batches was 6.7% -8.1%. The specificity of the pentachloronitrobenzene monoclonal antibody was relatively good, and the cross-reaction rates with pentachlorophenol, hexachlorobenzene, tetrachlorophthalide, and chlorothalonil were low, all of which did not exceed 30%. The ELISA kit could be stored at 4 °C for 12 months, showing good stability. [Conclusions]The detection kit has low cost, short time and small deviation, and is an ideal preliminary screening method.
- Subjects
WHITELEG shrimp; STANDARD deviations; IMMUNOASSAY; HEXACHLOROBENZENE; DETECTION limit; ENZYME-linked immunosorbent assay; MONOCLONAL antibodies
- Publication
Agricultural Biotechnology (2164-4993), 2021, Vol 10, Issue 5, p17
- ISSN
2164-4993
- Publication type
Article