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- Title
Exploring the idiotypes of insulin antibodies as markers for remission in Type 1 diabetes.
- Authors
Devendra, D.; Galloway, T.S.; Horton, S.J.; Wilkin, T.J.
- Abstract
Aims: Complete or partial remission can occur in newly diagnosed Type 1 diabetes patients. We created idiotype-specific reagents to explore the idiotypes of insulin antibodies (IA) in a patient in remission, and to compare with a patient who was not. Methods Phage display was used to create a library of phagotopes specific to insulin binding in four sera. Sera from a Type 1 diabetes subject deemed to have undergone remission were taken at diagnosis and again during remission. Sera from a non-remitter were taken at diagnosis and after 3 months on insulin. Phagotopes from the four sera were randomly selected and tested for insulin specificity in a radiobinding assay by using sera from remitters and non-remitters. Results IA-binding phagotope selected from serum during remission displaced insulin binding in all nine IA+ remitters and all 10 IA+ non-remitters. IA-binding phagotope selected from the non-remission patient (3 months alter insulin therapy) displaced insulin binding in 8/9 IA+ remitters and 8/10 IA+ non-remitters. The consensus peptide sequences adduced from the phages were identical for both these phagotopes. Phagotopes derived from insulin autoantibody-positive individuals at diagnosis were unable to displace insulin binding in the IA+ sera 3 months later, whether in remission or not. Conclusions We have established the principle of using phage display in the investigation of insulin antibodies during remission in Type 1 diabetes. The immunological characteristics of IA 3 months after the introduction of insulin treatment were different from those at diagnosis of Type 1 diabetes (IAA). Using phage display technology, it was not possible to distinguish insulin antibodies according to remission status.
- Subjects
INSULIN; IMMUNOGLOBULINS; BIOMARKERS; DIABETES; IMMUNOGLOBULIN idiotypes; DIAGNOSIS
- Publication
Diabetic Medicine, 2004, Vol 21, Issue 12, p1316
- ISSN
0742-3071
- Publication type
Article
- DOI
10.1111/j.1464-5491.2004.01344.x