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- Title
In vitro gene expression analysis of hepatotoxic drugs in rat primary hepatocytes.
- Authors
Suzuki, Hiromi; Inoue, Tomoaki; Matsushita, Tomochika; Kobayashi, Kazuko; Horii, Ikuo; Hirabayashi, Yoko; Inoue, Tobru
- Abstract
The article presents a study which examines the feasibility of screening for hepatoxicity by an in vitro gene expression analysis using rat primary hepatocytes and Affymetrix Rat Toxicology U34 arrays. It suggests that in hierarchical cluster analysis, drugs formed clusters depending on their mode of toxicity against cells. It notes that the number of transcripts affected by the cholestatic hepatotoxicants or the drugs that rarely cause hepatotoxicity were limited compared with other drugs where they did not induce transcriptional changes apparently related to toxicity. It concludes that in vitro gene expression analysis of hepatocytes using microarray is a useful tool for evaluating the toxicological profile of drugs in screening for the direct toxicity of drugs against hepatocytes.
- Subjects
IN vitro toxicity testing; GENETIC research; GENE expression; GENETIC regulation; CLUSTER analysis (Statistics); MEDICAL research; HEPATOTOXICOLOGY; PHARMACOLOGY; LABORATORY rats
- Publication
Journal of Applied Toxicology, 2008, Vol 28, Issue 2, p227
- ISSN
0260-437X
- Publication type
Article
- DOI
10.1002/jat.1328