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- Title
Inhibition kinetics on carboxylesterase and acetylcholinesterase of Liposcelis bostrychophila and Liposcelis entomophila (Psocop., Liposcelididae) of two insecticides.
- Authors
Cheng, W.-X.; Wang, J.-H.; Ding, W.; Zhao, Z.-M.
- Abstract
The inhibition kinetics on carboxylesterase (CarE) and acetylcholinesterase (AChE) of Liposcelis bostrychophila and Liposcelis entomophila of paraoxon and carbosulfan were compared. The results showed that L. entomophila exhibited significantly greater specific activity of CarE than L. bostrychophila [0.045 vs. 0.012 μmol of α-naphthyl acetate ( α-NA) hydrolysed/mg protein/min]. Moreover, L. entomophila showed higher affinity (i.e. lower Km value) to the substrate α-NA than L. bostrychophila (0.29 vs. 0.67 m m). For AChE-specific activity and affinity, no significant differences between the two species were observed. Based on the I50 values, AChE of L. bostrychophila was more sensitive to paraoxon and carbosulfan than that of L. entomophila. According to inhibition kinetics, the results revealed that AChE of L. bostrychophila was 3.8-fold more sensitive to inhibition by paraoxon than that of L. entomophila, but L. entomophila was 1.5-fold more susceptible to carbosulfan.
- Subjects
ENZYME kinetics; ACETYLCHOLINESTERASE; ENZYME inhibitors; CHEMICAL kinetics; CHOLINESTERASES; INSECTICIDES
- Publication
Journal of Applied Entomology, 2004, Vol 128, Issue 4, p292
- ISSN
0931-2048
- Publication type
Article
- DOI
10.1111/j.1439-0418.2004.00846.x