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- Title
Preliminary molecular detection of the somatic embryogenesis receptor-like kinase (<italic>VpSERK</italic>) and knotted-like homeobox (<italic>VpKNOX1</italic>) genes during in vitro morphogenesis of <italic>Vanilla planifolia</italic> Jacks.
- Authors
Ramírez-Mosqueda, Marco A.; Iglesias-Andreu, Lourdes G.; Sáenz, Luis; Córdova, Iván
- Abstract
This work aimed to evaluate the embryogenic competence of different tissues from different stages (friable callus, bud-regenerating callus, and whole buds) of <italic>Vanilla planifolia</italic>, through the molecular detection of the somatic embryogenesis receptor-like kinase (<italic>VpSERK</italic>) and knotted-like homeobox (<italic>VpKNOX1</italic>) genes. RNA was extracted with Trizol®, cDNA was obtained, and the studied transcripts were amplified. Using non-specific primers, <italic>VpSERK</italic> and <italic>VpSTM</italic> gene expression was detected in the three stages evaluated. This study might contribute to providing an explanation for the recalcitrance of this <italic>Vanilla</italic> species to somatic embryogenesis.
- Subjects
SOMATIC embryogenesis; KINASES; HOMEOBOX genes of plants; GENE expression in plants; PLANT morphogenesis
- Publication
3 Biotech, 2018, Vol 8, Issue 2, p0
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-018-1112-7