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- Title
Cell and Molecular Biology, Biochemistry and Molecular Physiology. Iron deficiency differently affects peroxidase isoforms in sunflower.
- Authors
Ranieri, Annamaria; Castagna, Antonella; Baldan, Barbara; Soldatini, Gian Franco
- Abstract
The response of both specific (ascorbate peroxidase, APX) and unspecific (POD) peroxidases and H[sub 2]O[sub 2] content of sunflower plants ( Helianthus annuus L. cv. Hor) grown hydroponically with (C) or without (-Fe) iron in the nutrient solution were analysed to verify whether iron deficiency led to cell oxidative status. In -Fe leaves a significant increase of H[sub 2]O[sub 2] content was detected, a result confirmed by electron microscopy analysis. As regards extracellular peroxidases, while APX activity significantly decreased, no change was observed in either soluble guaiacol or syringaldazine-dependent POD activity following iron starvation. Moreover, guaiacol-dependent POD activity was found to decrease in both ionically and covalently-cell-wall bound fractions, while syringaldazine-POD activity decreased only in the covalently-bound fraction. At the intracellular level both guaiacol-POD and APX activities underwent a significant decrease. The overall reduction of peroxidase activity was confirmed by the electrophoretic separation of POD isoforms and, at the extracellular level, by cytochemical localization of peroxidases by diaminobenzidine staining. The electrophoretic separation, besides quantitative differences, also revealed quantitative changes, particularly evident for ionically and covalently-bound fractions. Therefore, in sunflower plants, iron deficiency seems to affect the different peroxidase isoenzymes to different extents and to induce a secondary oxidative stress, as indicated by the increased levels of H[sub 2]O[sub 2]. However, owing to the almost completely lack of catalytic iron capable of triggering the Fenton reaction, iron-deficient sunflower plants are probably still sufficiently protected against oxidative stress.
- Subjects
SUNFLOWERS; HYDROGEN content of iron; PHYSIOLOGY
- Publication
Journal of Experimental Botany, 2001, Vol 52, Issue 354, p25
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/52.354.25